4t1乳腺癌細胞
細胞名稱：4t1乳腺癌細胞 小鼠乳腺癌細胞
細胞簡稱：4T1
產品貨號：TCM-C705
種屬來源：小鼠
組織來源：乳腺
特征：乳腺癌
細胞形態：上皮細胞樣
生長特性：貼壁生長
培養體系：RPMI-1640+10%FBS+1%P/S
4t1乳腺癌細胞培養基貨號：TCM-G705
傳代比例：1:3-1:6，每2-3天換液一次
傳代周期：48-72 h
培養條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存
質量檢測：細菌、真菌、支原體檢測均為陰性
4T1是從410.4瘤株中未經誘變篩得的6-硫鳥嘌噙抗性細胞株。當注射到BALB/c小鼠中時，4T1自發產生高轉移腫瘤，可轉移到肺，肝，淋巴結和大腦，同時在注射部位形成始發灶。誘導轉移時不需要摘除始發灶。4T1細胞在BALB/c小鼠中的生長與轉移特性與人體中的乳腺癌十分相近。這種腫瘤是人VI期乳腺癌的動物模型。4T1-誘導的腫瘤在手術后及未手術情況下轉移的動力學相近，可以用作手術后及未手術模型。跟其他腫瘤模型相比，由于4T1的抗6-硫鳥嘌噙特性，微小的轉移細胞團(少到僅僅1個)也可以在許多遠端器官中檢測到。
參考文獻：
Pulaski BA, et al. Immunotherapy with vaccines combining MHC class II/CD80+ tumor cells with interleukin-12 reduces established metastatic disease and stimulates immune effectors and monokine induced by interferon gamma. Cancer Immunol. Immunother. 49: 34-45, 2000. PubMed:
Pulaski BA, Ostrand-Rosenberg S. Reduction of established spontaneous mammary carcinoma metastases following immunotherapy with major histocompatibility complex class II and B7.1 cell-based tumor vaccines. Cancer Res. 58: 1486-1493, 1998. PubMed: 9537252
Pulaski BA, et al. Cooperativity of Staphylococcal aureus enterotoxin B superantigen, major histocompatibility complex class II, and CD80 for immunotherapy of advanced spontaneous metastases in a clinically relevant postoperative mouse breast cancer model. Cancer Res. 60: 2710-2715, 2000. PubMed:
Aslakson CJ, Miller FR. Selective events in the metastatic process defined by analysis of the sequential dissemination of subpopulations of a mouse mammary tumor. Cancer Res. 52: 1399-1405, 1992. PubMed: 1540948
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